Allele frequencies and allele lengths. 

Methods employed in population genetics studies are usefull to determine information on the genetic composition of heterogenous crop populations.

GCP developed the program FTOL-R to estimate allele frequencies for fingerprinting and genetic diversity studies, using bulked heterogeneous populations. The FtoL-R Program estimates the allele lengths of “dummy” data set of individuals, using the frequencies of marker-allele combinations from bulk populations.

The FREQS-R Program estimates allele frequencies for fingerprinting and genetic diversity studies using bulked heterogeneous populations.
FREQS-R  converts peak heights of SSR alleles run on a DNA sequencer into frequencies of the alleles within the bulk of individuals amplified with the SSR.  FREQS-R is a freely available program written using the R software. It was written as a simplified R version of the MSSC - SAS®/IML program (Dubreuil et al. 2005; Franco et al. 2005).
Find here the user's manual for the applicable for the FREQ-S program.
The freely available R version of the FREQ-S program can be found here. The FREQS-R program must be copied as a script into the R default directory and called using icons at Windows. 

The FtoL-R program was written to convert frequencies of SSR alleles run on an DNA sequencer for bulks of n individuals into length of alleles of n simulated individuals, hypothetically belonging to the bulk.  The allele frequencies of the hypothetical individuals are the same as the allele frequencies of the bulk. The FtoL program generates a “dummy” data set consisting of allele lengths for hypothetical individuals. These individuals correspond to bulk populations and are generated using the frequencies of marker-allele combinations from the bulks.
Find here the user's manual for the applicable for FtoL analysis progam.
The freely available R version of the FtoL program can be found  here. This FtoL-R program must be copied as a script into the R default directory, and generally called using icons in Windows

 

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