Genotyping Support Service Workshop--Zaragoza, Spain, 2007

 

From 24-28 September seven invitees from around the world participated in the Genotyping Support Service workshop in Zaragoza, Spain. The purpose of the workshop, which was organised by GCP in collaboration with Instituto Agronómico Mediterráneao de Zaragoza (IAMZ), was to facilitate participants with the analysis and interpretation of data as submitted in the pilot phase proposals, as well as assisting them with the preparation of plans to implement acquired knowledge into their current breeding activities. Overall coordination were handled by Humberto Gomez (GCP GSS Coordinator), Laura Ruiz (SP5 Programme Assistant) and Antonio López Franco (Cooperative Research Administrator at IAMZ). The meeting facilitated by Fred van Eeuwijk, Marcos Malosetti and Hans Jansen from Wageningen University.

Activities carried out by the seven workshop invitees included:

 

Molecular characterisation of Philippine Musa cultivars, by Emma Sales, University of Southern Mindanao, The Philippines
Emma Sales, who examined population structure of her Musa data, successfully explored the possibility of finding evidence to support a novel botanical classification for some of her Musa accessions. She also found indications that the morphological classifications of some of her germplasm need to be redone. More

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Molecular characterisation of banana germplasm accessions, by Alberto Vilarinhos Embrapa Cassava & Tropical Fruits
Alberto Vilarinhos, who also focused on the population structure of Musa data, looked for the identification of duplicates in his germplasm, and algorithms to find small sets of markers able to discriminate the largest number of accessions. More

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Discovery of new sources CMD resistance in cassava, by Emmanuel Okogbenin, National Root Crops Research Institute, Nigeria
Emmanuel Okogbenin studied his hypothesis of having found a new genetic source of resistance to Cassava Mosaic Disease. He concluded that he has two markers significantly associated with new sources of CMD resistance, and was able to determine TMS30555 and NR8083 as the likely sources. More

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Marker-assisted selection and participatory improvement of local cassava varieties for farmers preferred traits in Tanzania, by Heneriko Kulembeka, ARI, Tanzania, assisted by Cesar Ospina, CIAT, Colombia
Heneriko Kulembeka, together with Cesar Ospina, studied the results of Marker-Assisted Breeding data for sets of Tanzanian Cassava germplasm. They were able to identify a good combination of parents for CMD resistance and to learn the use of statistical software for that purpose. This team concluded that the 1st year of MAS in the field allowed spotting resistant parents much faster than with conventional approaches. Dr. Kulumbeka also became aware of the need to ensure good layout in future trials for more efficient collection of phenotypic data and for improved statistical analysis. He found supporting evidence to modify their breeding program by making more crosses from parents that combine both CMD and CBSD resistance (egAR30-3 x Namikonga, AR42-4 x Namikonga). Please click here for an abstract by Heneriko Kulembeka, and here for an abstract by Cesar Ospina

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Genotyping genetic resistance to the potato tuber moth (Phthorimaea oporcullela) in the tetraploid segregating families RDG116 (ND4382-19 x N142-72) and RD11 (P161-8 x Yagana) using a diversity array DArT made for this purpose, by Boris Sagredo, Instituo de Investigaciones Agropecuarias Centro Regional de Investigación (IIAM, Chile)
Boris Sagredo was interested in mapping insect resistance traits in potato segregating populations. He found more effective statistical means to handle phenotypic data from experiments of force feeding larvae in potatoes, generated in different from different assays. He found different responses of insect resistance in the different populations, and was able to map these quantitative responses. More

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Analysing potato and groundnut genetic diversity, by Jorge A. Rojas Beltran, PROINPA Foundation, Bolivia
Jorge Rojas analysed the population structure of a Bolivian potato collection. He was able to determine the genetic structure of the collection, and concluded that the genetic structure did not seem to be associated to geographical distribution. These findings will help Dr. Rojas to refine his plans to design core collections, which is a major institutional goal. Some accessions did not fall inside their expected species group, so their taxonomic discrimination will be revised. In addition, Dr Rojas located two potential duplicates in their germplasm. He also analysed a data set of groundnut germplasm collected in Bolivia, and was able to detect relatively large genetic variation and determine their genetic structure. Thanks to the genotyping data and its analysis, he expects to be able to offer a more effective support to the groundnut and potato breeding efforts of his institution. More

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